Fig 1: Kaplan–Meier curves for gastric cancer cases separated by USP44 expression. (A) Progression-free survival (PFS) and (B) overall survival (OS) curves for the low USP44 expression group (solid line) and high USP44 expression group (dotted line) (all cases, n = 207). (C) PFS and (D) OS in subgroups according to USP44 expression among diploid cases (n = 83). (E) PFS and (E) OS in subgroups according to USP44 expression among aneuploid cases (n = 124). P-value was calculated using the log-rank test.
Fig 2: Immunohistochemistry of USP44 expression in human gastric cancer clinical samples. Immunohistochemical detection of USP44 in human gastric cancer specimens was performed. Representative low USP44 expression (A) and high USP44 expression cases (B) are shown. (C) Immunohistochemical detection of USP44 in normal mucosa (left) and cancer (right) within the same case. (D) Bar graphs showing the individual proportion of positive nuclei in the normal mucosa (n = 85) and (E) cancer cases (n = 207).
Fig 3: Overexpression of USP44 leads to aneuploidy. (A) USP44 mRNA expression levels in control hTERT-RPE1 (n = 9), USP44-1 (n = 3), USP44-2 (n = 3), and USP44-3 (n = 3) cell lines were measured using qRT-PCR and standardized by ß-actin mRNA levels. *P < 0.0001. (B) Western blot analysis of USP44 protein expression in control hTERT-RPE1 cells and the three USP44 stably overexpressing cells was performed after 30 generations. ß-actin served as loading control. (C) Representative images of chromosomes of single cell samples were shown. Diploidy (left) and aneuploidy (middle, loss; right, gain) are shown. (D) Proportion of cells with aneuploidy was determined after 30 generations in control and USP44 overexpression cells. Data are means ± SD from three independent experiments (>50 cells per experiment). (E) Individual chromosome numbers from chromosome spreads were determined (total cell number: control, n = 204; USP44, n = 223). The normal chromosome number of hTERT-RPE1 cells is 46.
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